The kit supplies reagents used in microbiology for the research of acid-alcohol resistant Bacillus (BAAR) in biological materials. With this method, in addition to the bacteria belonging to the Mycobacteriaceae family, other germs with the same characteristic of alcohol-resistance are highlighted (Nocardia spp., Rhodococcus spp., Corynebacterium spp., Gordonia spp., Tsukamurella spp.).